In 1885, Abigail Wilson participated in an archaeological excavation at Rome's Colosseum. Fifteen years later, now a distinguished archaeologist and one half of the Museum Detectives with her husband, Daniel, she returns to Rome as a speaker at the Conference on Ancient Roman Civilization. Their visit takes a perilous turn when a fellow archaeologist and his wife are discovered murdered within the Colosseum, propelling the Wilsons into a complex investigation amidst the Eternal City's historic backdrop.